Abstract
A rapid method for qualitative and quantitative assaying of l‐lactate in milk is described. The method is based on oxidation of lactate by lactate oxidase with the simultaneous formation of H2O2 which subsequently oxidises 2, 4, 6‐tribromo 3‐hydroxy benzoic acid (TBHBA) in the presence of peroxidase. Oxidised TBHBA then reacts with 4‐amino antipyrine to obtain magenta colour (λmax = 523 nm). In the qualitative assay, colour change becomes visible in raw milk samples spiked with 300 mg lactate/L. The absorbance increases linearly up to 1000 mg/L. The method can be used for rapid assessment of the hygienic quality of milk.
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