Abstract
Objective: Development of a rapid isolation method to afford the bioactive metabolite (proximadiol: prox) from its herb (Cymbopogan proximus Stapf.) in the purest form and validate an LC method for its determination was our goal in this work. Methods: Prox was isolated by chromatographic techniques from the dichloromethane extract of the herb on alumina column, using petroleum ether-ethyl acetate solvent mixtures with increasing polarity.The determination of prox was achieved by LC method. The chromatographic separation was carried out on Thermo Hypersil ODS (4.5 x 250 mm, 10 µm) column, in the presence of 8-chlorotheophylline as an internal standard. The mobile phase was composed of 0.1 M phosphate buffer (pH: 3.5): methanol (60:40 v/v) and was pumped at a flow rate of 1 ml/min. The detection and quantification were done at 210 nm.Results: The structure elucidation of the isolated compound was identified on the basis of its spectral data.A simple and reproducible LC method was developed for the determination of the isolated purified Prox. Adequate separation and good resolution were obtained between prox and 8-chlorotheophylline. Quantification was achieved at 210 nm over concentration range 12.00-33.60 µg/ml with mean percentage recovery of 99.29±0.340. The method was validated in accordance with USP specifications. All analytical criterions were within acceptable range. The validity of the results was assessed by applying standard addition technique. The results obtained were compared with the reported method.Conclusion: The proposed method could be applied for routine quality control analysis of pharmaceutical formulations containing prox.
Highlights
Since the dawn of time, mankind has inherited the traditional use of medicinal plants in relieving pains and curing diseases
A simple and reproducible liquid chromatography (LC) method was developed for the determination of the isolated purified Prox
The proposed method could be applied for routine quality control analysis of pharmaceutical formulations containing prox
Summary
Since the dawn of time, mankind has inherited the traditional use of medicinal plants in relieving pains and curing diseases. Despite their side effects, they persist as traditional medicine in developing countries [1]. Most times the herbal products are introduced into the market with no suitable analytical evaluation. Consumers can buy such products without a physician's prescription and no one can determine the potential hazards in a lower grade product [2, 3]
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
More From: International Journal of Pharmacy and Pharmaceutical Sciences
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.