Abstract
A rapid method for the isolation of DNA from mouse cells grown in tissue culture is described. Cells are lysed in 0.24 m sodium phosphate, pH 6.8 buffer, containing 1% sodium dodecyl sulfate, 8 m urea, and 10 −3 m ethylene-diaminetetraacetic acid, and the crude lysate applied to a column of hydroxyapatite. RNA and proteins are removed from the column with 0.24 m sodium phosphate buffer containing 8 m urea while DNA is selectively eluted with 0.48 m sodium phosphate buffer. There is almost total recovery of cellular DNA from the column and the DNA is virtually free from proteins and RNA. This extraction procedure indicates that a mouse cell contains about 8 × 10 −6 μg of DNA.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
