Abstract
An in vitro regeneration system has been developed for Potentilla fulgens, which is an important Himalayan medicinal herb. Axillary shoot proliferation through shoot tip culture has been achieved on Murashige and Skoog (MS) medium containing 1mg l−1 6-benzylaminopurine (BAP) and 1 mg l−1 indole-3-acetic acid (IAA). Continuous production of plantlets with better rate of shoot multiplication and elongation obtained on MS medium supplemented with 1mg l−1 kinetin (Kin) alone or combined with 1mg l−1 α-napthaleneacetic acid (NAA). Established plantlets were successfully transferred to soil in a green house. The procedure ensures 12-fold plantlet production every 6 weeks.
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