Rapid in vitro propagation of Clematis heynei M. A. Rau: An important&lt;br&gt;medicinal plant

Jaykumar Chavan,Parthraj Gaikwad

doi:10.9755/ejfa.v24i1.10601

Abstract

The propagation of Clematis species has been studied for last 2-3 decades, but problems related to rooting havelimited the conventional methods to fulfil commercial needs. The present investigation describes an efficientmicropropagation protocol for Clematis heynei, an important medicinal plant. Multiple shoot production wassuccessfully achieved from axillary buds using nodes as explants. The highest organogenic response wasobtained from the nodal segments cultured on MS medium enriched with Kinetin (3 mgL-1) and indole-3-aceticacid (0.4 mgL-1). Microshoots were transferred for rooting in variety of auxins and its concentrations; in whichmaximum root induction was occurred in Indole-3-acetic acid (1 mgL-1). Through sequential hardening process,well-rooted plantlets were established in the field conditions and exhibited 88 % survival with normalmorphology and growth characteristics. This protocol is a successful and efficient biotechnological approach tothe micropropagation of C. heynei.

Highlights

Plants are valuable source of a vast array of chemical compounds
In view of its medicinal importance and the lack of tissue culture reports, the present study reports the prime protocol for regeneration of C. heynei from nodal explants
The morphogenic response of nodal explants of Clematis heynei was shown in almost all the treatments of BA, TDZ KN and Indole-3-acetic acid (IAA) (Table 1)

Summary

Introduction

Plants are valuable source of a vast array of chemical compounds. They synthesize and accumulate extractable organic substances in quantities sufficient to be economically useful as raw materials for various commercial applications. Industrialization coupled with urbanization is constantly putting pressure on natural resources. Due to depletion of habitat and ruthless collection, medicinal plants are on the verge of extinction. The conservation of these valuable genotypes is imperative. Plant tissue culture technology holds great promise for micropropagation, conservation, and enhancement of the natural levels of valuable secondary metabolites and to meet pharmaceutical demands and reduce the in situ harvesting of natural forest resources (Bapat et al, 2008)

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Conclusion