Abstract
Fragaria vesca L. has become a model species for genomic studies relevant to important crop plant species in the Rosaceae family, but generating large numbers of plants from non-runner-producing genotypes is slow. To develop a protocol for the rapid generation of plants, leaf explants were compared to single axillary bud shoot explants, both from in vitro-grown Fragaria vesca seedlings, as sources of shoots for new plant production in response to benzyladenine (BA) or thidiazuron (TDZ) combined with indolebutyric acid (IBA) on Murashige and Skoog’s Basal Salt (MS) medium. BA at 2.0 and 4.0 mg L−1 and TDZ at 1.5 mg L−1 promoted the greatest number of shoots produced per shoot explant. There were no IBA effects or IBA interactions with BA or TDZ. Significant interactions between BA and IBA, but not TDZ and IBA, occurred in leaf explant callus formation and % explants with callus at 6 and 9 weeks of culture and on shoots per leaf explant at 9 weeks. TDZ treatments produced uniformly high levels of callus but low numbers of shoots. The treatment generating the most shoot production was BA at 4.0 mg L−1 plus IBA at 0.50 mg L−1. After 9 weeks of culture, leaf explants of the non-runner-producing genotype Baron Solemacher had generated 4.6 shoots per explant with the best treatment, while axillary bud explants had generated 30.8 shoots with the best treatment. Thus, in vitro culture of shoot axillary bud explants can generate high numbers of clonal shoots from a single seedling plant in vitro.
Highlights
Fragaria vesca is a self-pollinating diploid species that has become a model species for the commercial strawberry
Preliminary work indicated that BA at 2 mg L−1 plus indolebutryic acid (IBA) at
0.125 mg L−1 had no effect compared to BA alone, and IBA concentration up to
Summary
Fragaria vesca is a self-pollinating diploid species that has become a model species for the commercial strawberry Most Fragaria species produce clonal plants on stolons (commonly called runners) that develop from axillary buds [5], but there are some important non-runnering genotypes within F. vesca [6], notably the F. vesca semperflorens, that flower constantly, with progeny that are primarily seed-derived and do not produce any runners (stolon, vegetative self-propagating unit). Studies of in vitro micropropagation of F. vesca have successfully regenerated shoots from leaf and petiole explants using combinations of benzyladenine (BA) and indolebutryic acid (IBA) following. F. vesca leaf explants placed abaxial side up regenerated shoots more rapidly than those placed adaxial side up after an Agrobacterium transformation treatment [11]
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