Abstract
The root systems of 11-wk-old Pinus densiflora seedlings were inoculated with a hyphal suspension of Tricholoma matsutake and aseptically incubated for 4 wk in a forest soil without supplying exogenous carbohydrates. One week following inoculation, fungal hyphae had colonized the root surface and bound soil particles together establishing a rootsubstrate continuum. Fungal hyphae were visible within the main root cortex following clearing, bleaching and staining. In the ensuing days, fungal colonization was observed within elongating lateral roots in which Hartig net formation was confirmed 4 wk after inoculation. This is the first report of rapid ectomycorrhizal infection of P. densiflora seedlings by T. matsutake.
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