Abstract
Hundreds of RNA binding proteins posttranscriptionally regulate gene expression, but relatively few have been characterized in plants. One successful approach to determine protein function has been to identify interacting molecules and the conditions of their association. The ribonucleoprotein immunopurification (RIP) assay facilitates the identification and quantitative comparison of RNA association to specific proteins under different experimental conditions. A variety of molecular techniques can be used to analyze the enriched RNAs, whether few as in the case of highly specific interactions, or many. Identification of associated RNAs can inform hypothesis generation about the processes or pathways regulated by the target protein. Downstream analysis of associated RNA sequences can lead to the identification of candidate motifs or features that mediate the protein-RNA interaction. We present a rapid method for RIP from tissues of plants that is suitable for experiments that require immediate tissue cryopreservation, such as monitoring a rapid response to an environmental stimulus.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.