Abstract
Molecular markers enable the detection and classification of fungi isolated from their natural environments. To develop species-specific markers for detecting Trichoderma koningiopsis and T. longibrachiatum, the sequence-characterized amplified region technique, using 20 inter-simple sequence repeat-polymerase chain reaction primers, was performed. The two specific markers for amplifying a single unique band consistent with T. koningiopsis and T. longibrachiatum, which were absent with other Trichoderma strains, were successfully identified. These fragments had no meaningful sequence homology with known sequences available in the National Center for Biotechnology Information and TrichOKEY databases. Compared with traditional identification techniques, these markers can facilitate more rapid and less complicated studies of Trichoderma population dynamics and evaluate their establishment after release into agricultural environments.
Highlights
Trichoderma, first described over 200 years ago, is a fungal genus including more than 200 species that are found globally in different geographical regions and climate zones (Atanasova et al 2013)
RAPD analysis was used to identify unique polymerase chain reaction (PCR) products that can be converted to Sequence-characterized amplified region (SCAR) markers for filamentous fungal species or strains of interest (Parmar et Hassan et al Egyptian Journal of Biological Pest Control (2019) 29:13 al. 2015b)
The inter-simple sequence repeat (ISSR)-based SCAR-PCR protocol is superior to existing Aspergillus section Flavi detection systems because of its simplicity and minimal sample handling requirements Priyanka et al (2014)
Summary
Trichoderma, first described over 200 years ago, is a fungal genus including more than 200 species that are found globally in different geographical regions and climate zones (Atanasova et al 2013). Numerous molecular techniques have been used for Trichoderma identification to investigate the genetic diversity within the genus These include restriction fragment length polymorphism (Dodd et al 2004), random. To improve upon these previous methods, a simple marker system must enable rapid and inexpensive species identification. RAPD analysis was used to identify unique PCR products that can be converted to SCAR markers for filamentous fungal species or strains of interest (Parmar et Hassan et al Egyptian Journal of Biological Pest Control (2019) 29:13 al. Yuantian et al (2015) established a simple and reliable strain diagnostic system for T. harzianum, using a PCR-based technique They first analyzed T. harzianum, using RAPD analysis, to assess its genetic diversity and produce a strain-specific molecular marker. SCAR markers have been used to identify different species and biotypes of the Asian gall midge (Behura et al 1999), a major insect pest of rice, and for the specific identification of six Trypanosoma cruzi lineages (Skoneczny et al 2015)
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