Abstract
A simple and rapid method using real-time PCR and melting temperature analysis is described for the detection of the origin of the matrix gene in reassortant influenza A viruses. This procedure relies on differences between the matrix gene PCR product melting temperature of recent human A(H1N1) and A(H3N2) strains and the laboratory donor strain PR-8 allowing the origin of the matrix gene to be identified. This novel method offers advantages over previous methods such as hybridisation and RFLP in facilitating rapid selection of candidate vaccine strains.
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