Abstract
Clinical treatment of the infections caused by various staphylococcal species differ depending on the actual cause of infection. Therefore, it is necessary to develop a fast and reliable method for identification of staphylococci. Raman spectroscopy is an optical method used in multiple scientific fields. Recent studies showed that the method has a potential for use in microbiological research, too. Our work here shows a possibility to identify staphylococci by Raman spectroscopy. We present a method that enables almost 100% successful identification of 16 of the clinically most important staphylococcal species directly from bacterial colonies grown on a Mueller-Hinton agar plate. We obtained characteristic Raman spectra of 277 staphylococcal strains belonging to 16 species from a 24-hour culture of each strain grown on the Mueller-Hinton agar plate using the Raman instrument. The results show that it is possible to distinguish among the tested species using Raman spectroscopy and therefore it has a great potential for use in routine clinical diagnostics.
Highlights
Background removal methoditerative polynomial fitting (IPF)Rolling-Circle Filter (RCF) accuracy [%] Nopt accuracy [%] Nopt 99.2−2 = two methods of fluorescence background removal (IPF and RCF)
The results show that it is possible to distinguish among the tested species using Raman spectroscopy and it has a great potential for use in routine clinical diagnostics
The fluorescent background was removed by the IPF method
Summary
Recent studies suggest that we can use Raman spectroscopy for the detection of antimicrobial resistance[48,66] and other virulence factors like the ability to form biofilms[37,41,43]. These studies support the prospective use of Raman spectroscopy as a tool for microbial diagnostics. This was already proven for yeast spectra acquired in a time window of more than one year and the findings were supported by our recent work on S. aureus and S. epidermidis[38,51]
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