RAPID IDENTIFICATION OF PASTEURELLA PESTIS BY MICROSCOPICAL OBSERVATION OF BACTERIOPHAGE LYSIS

Abstract

Strains of Pasteurella pestis, grown on Fildes" or sulphite agar, or on a selective medium, at 30 °C formed microcolonies containing from 20 to 50 bacilli in 5 to 6 hours. These microcolonies were treated with a P. pestis bacteriophage and subsequently incubated at 18 °C. Lytic changes in the microcolonies began about 3 hours after application of the phage and the microcolonies were largely destroyed 2 hours later. The phage lysed microcolonies of several strains of Pasteurella pseudotuberculosis, two of Escherichia coli, and one of Shigella dysenteriae at 30 °C but at 18 °C it was active only on E. coli K12 C600 besides P. pestis. The susceptible E. coli strain did not grow on the selective medium, which could therefore be used to increase the specificity of the test. Methods for performing this test in practice are discussed. The technique would allow P. pestis to be identified about 10 hours after inoculation of an agar plate with infected material. Although slower than a fluorescent antibody reaction performed on a direct smear of infected material, this phage-lysis procedure may find a place as a semirapid confirmatory test for P. pestis.