Abstract
Tubercle bacillus [TB] is one of the most important chronic infectious diseases that cause millions of deaths annually. While conventional smear microscopy and culture methods are widely used for diagnosis of TB, the former is insensitive, and the latter takes up to 6 to 8 weeks to provide a result, limiting the value of these methods in aiding diagnosis and intermediate decisions on treatment. Therefore, a rapid detection method is essential for the diagnosis, prognosis assessment, and recurrence monitoring. A new surface plasmon resonance [SPR] biosensor based on an array format, which allowed immobilizing nine TB antigens onto the sensor chip, was constructed. Simultaneous determination of multiple TB antibodies in serum had been accomplished with this array-based SPR system. The results were compared with enzyme-linked immunosorbent assay, a conventional immunological method. Array-based SPR showed more advantages in providing label-free and real-time detection. Additionally, the high sensitivity and specificity for the detection of TB infection showed its potential for future development of biosensor arrays for TB diagnosis.
Highlights
Mycobacterium tuberculosis [MTB] is the causative agent of tubercle bacillus [TB], accounting for approximately two million deaths annually, mainly in developing countries [1], and remains one of the leading causes of respiratory infections and has posed critical threats to public health [2]
We developed a new SPR-based biosensor for clinical antibody detection of TB diseases in a parallel manner for the first time
Injection of a serum sample of a TB patient, but not a healthy donor, caused an initial increase and the stabilization of the signal response corresponding to the spot bearing the TB antigen (Figure 2)
Summary
Mycobacterium tuberculosis [MTB] is the causative agent of tubercle bacillus [TB], accounting for approximately two million deaths annually, mainly in developing countries [1], and remains one of the leading causes of respiratory infections and has posed critical threats to public health [2]. The rapid detection method with high sensitivity and specificity is essential to aid the diagnosis, assess the prognosis, and monitor the disease recurrence [4]. EIA employing multiple antibody probes for bacteria detection leads to both the complexity and the cost of the method. There are still needs to develop better technologies that can reduce detection complexity and perform faster diagnosis while maintaining high sensitivity and specificity. A multiantigen print immunoassay has been recently employed for profiling multiple antibodies to tuberculosis [15]. This immunoassay requires longer incubation time to carry out the antibody-antigen interaction compared with other biosensors
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