Rapid Identification of Insecticide- and Herbicide-Tolerant Genetically Modified Maize Using Mid-Infrared Spectroscopy

Abstract

Genetically modified (GM) technology is of great significance for increasing crop production, protecting biodiversity, and reducing environmental pollution. However, with the frequent occurrence of safety events regarding GM foods, more and more disputes have arisen over the potential safety of transgenic technology. It is particularly necessary to find a fast and accurate method for transgenic product identification. In this research, mid-infrared spectroscopy, coupled with chemometric methods, was applied to discriminate GM maize from its non-GM parent. A total of 120 GM maize and 120 non-GM maize samples were prepared, and the spectral information in the range of 400–4000 cm−1 was collected. After acquiring the spectra, wavelet transform (WT) was used to preprocess the data, and k-means was carried out to split all samples into calibration and prediction sets in the ratio of 2:1. Principal component analysis (PCA) was then conducted to qualitatively distinguish the two types of samples, and an apparent cluster was observed. Since the full spectrum covered a large amount of data and redundant information, we adopted the successive projections algorithm (SPA) to select optimal wavelengths for further analysis. Chemometrics, including partial least squares-discriminant analysis (PLS-DA), the k-nearest neighbor algorithm (KNN), and the extreme learning machine (ELM), were performed to establish classification models based on full spectra and optimal wavelengths. The overall results indicated that ELM models based on full spectra and optimal spectra showed better accuracy and reliability, with a 100% recognition rate in the calibration set and a 98.75% recognition rate in the prediction set. It has been confirmed that mid-infrared spectroscopy, combined with chemometric methods, can be a novel approach to identify transgenic maize.