Rapid Identification of HSA Genetically Modified Goats by Combining Recombinase Polymerase Amplification (RPA) with Lateral Flow Dipstick (LFD)

Abstract

Genetically modified (GM) animals have attracted considerable attention and faced challenges. Human serum albumin (HSA) GM goats have been used to produce goat milk with serum albumin from humans, which has shown great potential in the market. Establishing an accurate method to distinguish goats with a genome modified by HSA has become necessary. Here, we established a platform to detect HSA GM goats by combining the advantages of recombinase polymerase amplification (RPA) and lateral flow dipstick (LFD) strategies. The whole detection process could be completed within 1 h, obtaining a direct result that could be visualized by a characteristic red band after a quick amplification under a constant temperature of 42 °C in the RPA experiments. Moreover, the GM goats could be identified with a detection limit of 0.1 ng using our method. Therefore, this study provided a rapid and convenient RPA-LFD method for the immediate detection of HSA GM goats. This will be useful for the identification of HSA GM goats, which may be used to distinguish the mixture of GM mutton and normal mutton.