Abstract
SummaryGrouper (Epinephelus marginatus) and wreck fish (Polyprion americanus) are two costly and demanding fish species which are subjected to substitution in the restaurant industry. In our study, 44 purported grouper and wreck fish meals (24 from school and university lunch rooms and 20 from restaurants) were analysed using an indirect enzyme‐linked immunosorbent assay (ELISA) (microtiter plates and immunostick format) and a MAb specific to these fish species. The results showed that 29 out of 44 meal samples were misdescribed in the restaurant menus. This ELISA procedure, which has proved to be specific, sensitive, cheap and very simple to develop, could be very useful for accurate authentication of meals in the restaurant industry.
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