Rapid Identification of Chemical Constituents in Hericium erinaceus Based on LC-MS/MS Metabolomics

Fei Yang,Lili Cui,Guoquan Feng,Jinmei Wang,Honglin Wang,Sulan Zhang,Xiao-Zhi Tang

doi:10.1155/2021/5560626

Abstract

Hericium erinaceus is a precious edible and medicinal fungus with high nutritional value. It has many functions, such as enhancing immunity, antitumor antioxidation, antihyperglycemic, antihyperlipidemic, and protecting gastric mucosa. However, there are few researches about the H. erinaceus compounds. In this paper, ultraperformance liquid chromatography tandem high-resolution mass spectrometry (UPLC-Q-exactive-MS/MS) was used to isolate and identify the compounds in H. erinaceus. 102 compounds were identified in H. erinaceus by comparing with standard databases such as MZVault, MZCloud, and BGI Library (self-built standard Library by BGI Co., Ltd), including flavonoids, terpenoids, phenolic acids, phenylpropanoids, steroids, organic acids, and amino acids.

Highlights

Hericium erinaceus is a precious edible and medicinal fungus, and it is listed as one of the “Four Famous Cuisines” of China, together with bear’s paws, trepang, and shark’s fin [1]; it has been used for a long time in traditional Chinese medicine [2]
Results of Compound Identification. e compounds of H. erinaceus were analyzed by LC-MS/MS; the identification of structure was based on the retention time, MS data, and MS2 data compared with the standard database. e identified compounds were classified into three grades (Level 1, Level 2, and Level 3) according to the comparison results, there into, Level 2 was confirmed on the basis of MS data, MS2 data, and properties of compounds, and Level 3 was identified on the basis of MS data and MS2 data. e credibility sequence is as follows: Level 1> Level 2> Level 3
Detailed results are shown in Table 1. 102 compounds were preliminarily identified in H. erinaceus with grade of identification as Level 1 and Level 2, including 31 organic acids, 10 nucleotides and analogues, 8 amino acids, 6 carbohydrates and derivatives, 5 flavonoids, 3 unsaturated fatty acids, 3 terpenoids, 3 phenolic acids, 1 phenylpropanoid, 1

Summary

Introduction

Hericium erinaceus is a precious edible and medicinal fungus, and it is listed as one of the “Four Famous Cuisines” of China, together with bear’s paws, trepang, and shark’s fin [1]; it has been used for a long time in traditional Chinese medicine [2]. 8 phenolics were isolated and identified from fruiting bodies of H. erinaceus by the Kawagishi team between 1990 and 1993, named Hericenone A-H [11–13]. Two new phenolics were isolated from fruiting bodies of H. erinaceus by Ma et al [15], named Hericenone I and Hericenone D; they have the same fatty acid chain. A new skeleton phenolic compound was isolated from fruiting bodies of H. erinaceus by Li et al [16], named Erinacene D. In addition to terpenoids and phenolics compounds, H. erinaceus was rich in steroids, six new (erinarols A-F), and five known ergostane-type sterol fatty acid esters were isolated from the fruiting bodies of H. erinaceus; erinarols A and B significantly activated the transcriptional activity of PPARs, PPARα, and PPARc [17]. Previous pharmacological studies showed that H. erinaceus had many pharmacological activities, such as regulating immunity [18, 19], neuroprotection [20, 21], antidepressant [22, 23], antioxidant [24], antitumor [25], antihyperglycemic [26], and antihyperlipidemic properties [27], and protecting gastric mucosa [28, 29]

Methods

Results

Conclusion