Abstract
150 clinical isolates of Candida species, Trichosporon species and Pichia anomala as well as 32 yeasts isolated from dairy products were tested in order to evaluate the utilization of a specific fluorogenic substrate in direct detection of Candida albicans. Detection of N- acetyl-β- d-galactosaminidase (NAGase) was performed with fluoroplate candida agar (Merck, Darmstadt, FRG), which contained 4- methylumbelliferyl-N- aceryl-β — d-galactosaminide (4-MUAG). The API 20C yeast identification system was used as a reference identification method for all isolates. The results of our investigations showed that 99% of C. albicans and all strains of C. stellatoidea examined were NAGase-positive. There were two false positive reactions of four C. tropicalis isolates and one false positive reaction of three Trichosporon beigelii strains.
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