Abstract
Potentilla freyniana Bornm. (P. freyniana), belonging to the family Rosaceae, has been used as a folk medicine in China. However, as we know, the constituents and the systematic elucidation of the mechanism were not fully investigated. Therefore, it is necessary to develop a rapid method using LC-MS and network pharmacology for the detection and identification of constituents and the systematic mechanism of P. freyniana. Firstly, the flavonoids were detected and identified based on ultra-high-performance liquid chromatography coupled with Quadrupole-Exactive Focus Orbitrap MS (UHPLC-Q-Exactive Orbitrap MS). After that, the potential targets of those constituents were obtained by database mining. Then, the core targets were predicted by protein-protein interaction network and network analysis. Finally, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis were carried out via DAVID. This finding revealed that P. freyniana possessed 43 flavonoids (40 of them were first reported) with 23 core target genes, which are associated with PI3K-Akt, MAPK, TNF signaling pathway, and pathway in cancer. This study demonstrated the multicompound, multitarget, and multimechanism of P. freyniana, which are very beneficial to develop the further study and utilization of this plant including the material basis and quality control research.
Highlights
Potentilla freyniana Bornm. (P. freyniana), a genus Potentilla of the family Rosaceae, named Difengzi, is a perennial plant with branched and tufted roots widely distributed and cultivated allaround of China, especially in Hunan, Hubei, Jiangxi. eir roots have been used as a folk medicine in clearing away heat and toxic materials for treating canker, bone tuberculosis, external bleeding [1,2,3]
P. freyniana possessed 43 flavonoids (40 of them was first reported) with 23 core target genes, which are associated with PI3K-Akt, MAPK, TNF signaling pathway, and pathway in cancer. is study demonstrated the multicompound, multitarget, and multimechanism of P. freyniana, which are very beneficial for the further study and utilization of this plant including the material basis and quality control research
Chemicals and Materials. e chemical reference standards isoquercitrin, luteolin, naringenin, and kaempferol were provided by Chengdu Herbpurify biotechnology CO., LTD (Chengdu, China); Phlorizin, Phloretin, and Trilobatin were purchased from Sichuan Wei Keqi Biotechnology Co., Ltd (Sichuan, China); eriodictyol and hyperoside were provided by Chengdu Push biotechnology CO., LTD (Chengdu, China); quercetin and apigenin were provided by Chengdu Alfa biotechnology CO., LTD (Chengdu, China); baicalein and wogonin were obtained from Chengdu Desite biotechnology CO., LTD (Chengdu, China). e purities of all chemical reference standards were above 98% by HPLC-DAD
Summary
Potentilla freyniana Bornm. (P. freyniana), a genus Potentilla of the family Rosaceae, named Difengzi, is a perennial plant with branched and tufted roots widely distributed and cultivated allaround of China, especially in Hunan, Hubei, Jiangxi. eir roots have been used as a folk medicine in clearing away heat and toxic materials for treating canker, bone tuberculosis, external bleeding [1,2,3]. Previous investigations on P. freyniana showed the presence of different compounds including terpenes and flavonoids [4,5,6,7] and possessed a variety of activities such as anti-inflammatory, analgesic effects [8, 9]. 14 compounds, including eriodictyol, phlorizin, were separated from the roots of P. freyniana [7]. Erefore, it is worthwhile to establish a highly sensitive method for characterizing their chemical constituents and elucidating systematic pharmacological mechanism of P. freyniana. E complexity of chemicals in Traditional Chinese Medicine (TCM) including P. freyniana has presented a significant challenge in the rapid identification and characterization of components. Erefore, this current study was designed to develop a fast and effective method for the chemical characterization and systematic pharmacological mechanism of P. freyniana using UHPLC-Q-Exactive Orbitrap MS and network pharmacology. P. freyniana possessed 43 flavonoids (40 of them was first reported) with 23 core target genes, which are associated with PI3K-Akt, MAPK, TNF signaling pathway, and pathway in cancer. is study demonstrated the multicompound, multitarget, and multimechanism of P. freyniana, which are very beneficial for the further study and utilization of this plant including the material basis and quality control research
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