Rapid, high sensitivity, point-of-care test for cardiac troponin based on optomagnetic biosensor

Abstract

BackgroundWe present a prototype handheld device based on a newly developed optomagnetic technology for the sensitive detection of cardiac troponin I (cTnI) in a finger-prick blood sample with a turnaround time of 5min. MethodsThe test was completed in a compact plastic disposable with on-board dry reagents and superparamagnetic nanoparticles. In our one-step assay, all reaction processes were precisely controlled using electromagnets positioned above and below the disposable. Nanoparticle labels (500nm) bound to the sensor surface via a sandwich immunoassay were detected using the optical technique of frustrated total internal reflection. ResultsA calibration function measured in plasma demonstrates a limit of detection (mean of blank plus 3-fold the standard deviation) of 0.03ng/mL cTnI. A linear regression analysis of the region 0.03–6.5ng/mL yields a slope of 37±4, and a linear correlation coefficient of R2=0.98. The measuring range could be extended substantially to 100ng/mL by simultaneously imaging a second spot with a lower antibody concentration. ConclusionsThe combination of magnetic particles and their fine actuation with electromagnets permits the rapid and sensitive detection of cTnI. Because of the potential high analytical performance and ease-of-use of the test, it is well suited for demanding point-of-care diagnostic applications.