Abstract
Lopinavir is a new specific and potent HIV-1 protease inhibitor. A rapid high-performance liquid chromatographic method using UV detection, has been developed and validated for the analysis of lopinavir in plasma. This involved a single liquid-solid extraction on an OASIS® HLB column in the presence of an internal standard. Separation was achieved on a Xterra®, C8 (150×3.9 mm I.D.) column with a mobile phase consisting of acetonitrile and water (41∶59, v/v). The detection wavelength was 210 nm. The assay was linear from 0.187 to 10.0 μg.mL−1 and the limit of quantification was 0.187 μg.mL−1. Mean recovery was ranged from 90.7% to 97.8% for lopinavir and 97.1% for the internal standard. Day to day precision and accuracy were less than 9.6% and 7.3% respectively. This rapid and simple method can readily be used for drug monitoring of lopinavir, in HIV-1 infected patients.
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