Abstract
In conventional latex-particle agglutination tests, latex beads are either coated with antibody and clump if antigen is present in a sample or are coated with antigen and clump if an antibody is present in a sample. Ligands other than antigen and antibody have not been used previously for latex-particle agglutination tests. We report here a new variation, a sandwich latex-particle agglutination test in which interactions between GM1 gangliosides, antigen (cholera toxin) and anticholera antibody are used to affect agglutination. the principles that are described can be applied to develop tests for the rapid detection of other small ligands.
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