Rapid Flow Injection Electrochemical Detection of Arochlor 1242 Using Stabilized Lipid Membranes with Incorporated Sheep anti‐PCB Antibody

Abstract

AbstractThis work describes a novel electrochemical biosensor based on a supported polymerized lipid film with incorporated Sheep anti‐PCB antibody for the rapid detection of arochlor 1242 in flowing solution streams. The antibody was incorporated into the lipid film during polymerization. Injections of antigen were made into flowing streams of a carrier electrolyte solution. Experiments were done in a stopped‐flow mode using lipid mixtures containing 15 % (w/w) dipalmitoylphosphatidic acid (DPPA) to provide only a single transient current signal with a magnitude related to the antigen concentration. Lipid films containing 35 % DPPA were used to examine regeneration of the active sites of antibody after complex formation by washing with the carrier electrolyte solution. Repetitive cycles of injection of antigen have shown that the maximum number of cycles is about 5.