Abstract
Rapid, exponential growth ofFrankia sp. BR (ORS 020608) isolated fromCasuarina equisetifolia was found to require stirring (magnetic bar) and adequate buffering (MES or MOPS buffers). We used a mineral medium (BAP) at 28°C with propionate and NH4+ as the sole carbon and nitrogen source respectively. Sporulation was enhanced by stirring, but the addition of phosphatidyl choline was found to impair sporulation and slightly improve growth. In these conditions a generation time of 14–16 hours was observed with a maximal biomass yield of 108 μg of protein × mL-1 (Bicinchoninic acid) at day 4 when growth ceased. In stirred conditionFrankia growth as microcolonies (30–200 μm diameter) showing an ‘open mesh’ type of mycelium and also a random distribution of growing hyphae inside and in the periphery of the microcolonies (acridine orange vital staining). In static conditions macrocolonies and only peripheric growth can be observed. Optimal growth in buffered and stirred conditions in mineral medium has been also observed with Cj and CeFFrankia isolates. The reasons for rapid and balanced hyphal growth in stirred conditions are discussed.
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