Abstract

Ethylene induces petal abscission in diploid geranium flowers. If the flowers are exposed to 1 µ1/L ethylene for 1 hour, petal abscission begins after about 60 minutes and is complete by 90 minutes from the start of the ethylene treatment. This response is inhibited by Actinomycin D, a transcriptional inhibitor, and by cycloheximide, an inhibitor of translation. The amount of total protein in abscission zone tissue doubles during the ethylene treatment. SDS gel electrophoresis of petal abscission zone proteins shows that there are some individual protein species which appear and some which disappear during ethylene treatment. Our hypothesis is that ethylene induces gene activation, and possibly repression, in abscission zone cells.

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