Abstract
Very rapid accumulation of free 1-aminocyclopropane-1-carboxylic acid (ACC), followed by stimulation of ethylene production were induced by a Cu2+ in soybean cuttings. The accumulation must be attributed to an increase in ACC synthesis, because: (1) it was completely inhibited by aminoethoxyvinylglycine (AVG); and (2) the ethylene stimulation was inhibited by AVG, indicating that free ACC cannot be released from its conjugated form. The activity of the ethylene-forming enzyme slightly decreased following the Cu2+ pulse, and this event was accompanied by a slight increase in electrolyte leakage from the treated soybean tissues.
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