Abstract
An attempt was made to perform high-speed gel filtration in 6 m guanidine hydrochloride. A column of silica-based aqueous gels, G 3000 SW or G 4000 SW, in conjunction with a high-pressure liquid chromatograph, gave satisfactory results under conditions where a single run was completed within 50 min. Further increase in speed was possible at some sacrifice of resolution. The reproducibility, resolving power, and sensitivity achieved, together with the linearity of calibration plots, have revealed that this method is exceedingly useful for the rapid estimation of the molecular weights of protein polypeptide chains.
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