Abstract
A rapid enzyme-linked immunosorbent assay (ELISA) was developed to evaluate sera from channel catfish with and without Edwardsiella ictaluri infection. Anti- E. ictaluri antibodies were detected in the sera from 94 of 100 infected catfish. Specific antibodies were not detected in the sera from 100 non-infected fish. The ELISA was based on the Falcon Assay Screening Test (FAST) and was performed in 30 min. An E. ictaluri exoantigen was used in the FAST-ELISA. The immunodominance of the exoantigen was shown by reactivity with monoclonal antibody (MAb) against the predominant epitope of E. ictaluri. The FAST-ELISA was found to be ideal for serodiagnosis and seroepidemiological purposes of detecting and monitoring E. ictaluri antibody responses.
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