Abstract
AbstractA simple method of rat brain embedding into egg yolk facilitates serial sectioning of the whole brain in coronal plane. The sections are compact and can easily be treated by such free floating techniques as silver impregnation or immunohistochemistry. Unembedded material is hard to process in this way, as caudal parts of the brain (including occipital cortices, cerebellar convolutions, and brain stem) form separate pieces that must be treated individually, and their unfolding and appropriate positioning onto slide is difficult. A simple and rapid embedding protocol greatly simplified processing the large number of sections. This technique may be of practical importance in several cases where frozen or vibratome brain sections are to be processed by floating technique. (The J Histotechnol 19:143, 1996)
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