Abstract
A rapid, efficient and high-performance transformation protocol employing Agrobacterium rhizogenes was developed for the common bean Phaseolus vulgaris. In this study, we examined competencies of various protocols to induce and explants that respond to hairy root transformation in bean plants. Utilizing young seedlings with severed radicles/hypocotyls, we developed a highly efficient procedure for achieving hairy root transformation frequencies as high as 100% as visualized by GUS reporter gene expression system. Transgenic hairy roots in these young composite plants were susceptible to nodulation by rhizobia, and form an excellent system for high throughput genomic analysis to study root biology and endosymbiosis in common bean.
Highlights
Leguminous plants are capable of entering into symbiotic association with soil bacteria commonly known asHow to cite this paper: Khandual, S. and Reddy, P.M. (2014) Rapid, Efficient and High-Performance Protocol for Agrobacterium rhizogenes-Mediated Hairy Root Transformation of the Common Bean Phaseolus vulgaris
The common bean P. vulgaris is a most widely cultivated and consumed crop legume in the developing world. It is a diploid plant with relatively a small genome (650 Mb), and consumed crop legume in the developing world
It is a diploid plant with relatively a small genome (650 Mb), potentially an ideal model plant from among crop legumes to study genetic basis of plant development including root nodule symbiosis
Summary
How to cite this paper: Khandual, S. and Reddy, P.M. (2014) Rapid, Efficient and High-Performance Protocol for Agrobacterium rhizogenes-Mediated Hairy Root Transformation of the Common Bean Phaseolus vulgaris. (2014) Rapid, Efficient and High-Performance Protocol for Agrobacterium rhizogenes-Mediated Hairy Root Transformation of the Common Bean Phaseolus vulgaris. P. vulgaris is a diploid plant with relatively a small genome (650 Mb), and forms an excellent model plant among crop legumes to study nodulation and nitrogen fixation. It has lagged behind the model legumes L. japonicus and M. truncatula in becoming an ideal system to study root symbiosis because of its recalcitrance to genetic transformation. Estrada-Navarrete et al [3] developed an Agrobacterium rhizogenes-mediated hairy root transformation protocol for common bean, and this paved way to further molecular studies on nodule development in this plant species. We examined the effectiveness of various protocols, explants and introduced appropriate amendments to boost hairy root transformation efficiencies to 100%, making it a suitable system for high-throughput functional genomic studies in bean
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