Abstract
An experimental study on droplet generation for the rapid detection of cells by using cell sorters was conducted. Cell sorters create micro-droplets by controlling waves at surface of a micro-jet which consists of samples and sheath fluid. In this paper the optimum wave number was observed by microscope. The ratio of sample flow rates to sheath flow rates and frequency of the wave are also controlled in order to investigate those effects on droplet generation. In addition to the experimental studies linear instability analysis for droplet generation and numerical simulations for the flow distribution in the chamber of the cell sorter were carried out to obtain optimum parameters to maximize the rate of droplet generation. Analytical solutions agree well with the experimental results of droplet generation.
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