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Abstract
Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) was applied to the rapid discrimination of four lactic acid bacteria (Lactobacillus acidophilus, Lactococcus lactis subsp. hordniae, and two strains of Lc. lactis subsp. lactis). Specific biomarker peaks generated from intact bacteria cell were observed on the mass spectra over the range m/z 4000∼12000. High reproducibility for the relative peak intensities between three culture plates under the same experimental conditions was confirmed by analysis of variance (ANOVA). The growth temperature strongly affected the peak distribution of the biomarkers, whereas the difference in the culture media (MRS and LB media) gave similar mass spectra. Under the optimized conditions, four lactic acid bacteria with different genus, species, or strains could be discriminated.
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