RAPID DISCRIMINATION AMONG METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS ISOLATES USING VARIABLE NUMBER TANDEM REPEAT ANALYSIS IN A SAMPLE OF IRAQI PATIENTS AND

A M Abd Zaid,N J Kandala

doi:10.36103/ijas.v52i5.1456

Abstract

There is so little information available in Iraq about genetic variability in methicillin resistant Staphylococcus aureus (MRSA), so current study aimed to use six tandem repeat loci of multilocus variable number tandem repeat (MLVA) typing to discriminate among MRSA, so to achieve the aim of this study, six loci, clfA, clfB, sdrC, spa, sspa and sav1078 were selected for multiplex PCR. The PCR product were subjected to capillary electrophoresis by using ABI-Genetic analyzer, then the data were analyzed by using GeneMapper™ Software 5. Fragment sizes were converted into repeats number. The total number of repeats are used to generate allelic profile. The allelic profile used to draw the minimum spanning tree and dendrogram , all the 85 MRSA isolates are clustered into 54 MLVA type.

Highlights

Staphylococcus aureus is linked nosocomial and community-acquired disease and it is considered an important bacterial pathogen [6]
In the past few years methicillin resistant S. aureus bacteria has been rapidly emerged due to excessive usage of methicillin within hospitals worldwide [27, 22, 25]
Multiplex PCR based multilocus variable number of tandem repeats (MLVA) assay The multiplex PCR was used for MLVA typing by using six genes included spa (Protein A gene), sdrC (Ser-Asp-rich fibrinogen binding proteins), clfA (Clumping factor A), clfB (Clumping factor B), sspa (Serine protease V8) and sav1078 (Hypothetical Protein)

Summary

INTRODUCTION

Staphylococcus aureus is linked nosocomial and community-acquired disease and it is considered an important bacterial pathogen [6]. A technique known MLVA which is multiplelocus variable-number tandem repeat analysis that knowing by its ability to typing bacterial pathogens in large number [4, 7, 8, 11, 19, 20, 21]. In Iraq, there is so little information available about genetic variability in MRSA regionally, so current study aimed to use six tandem repeat loci (sdrC, spa, clfB, clfA, sspA and sav1078) of MLVA typing to discriminate among MRSA. Multiplex PCR based multilocus variable number of tandem repeats (MLVA) assay The multiplex PCR was used for MLVA typing by using six genes included spa (Protein A gene), sdrC (Ser-Asp-rich fibrinogen binding proteins), clfA (Clumping factor A), clfB (Clumping factor B), sspa (Serine protease V8) and sav1078 (Hypothetical Protein).

Dye References

Temperature o

Isolates and phenotypic identifications

CONCLUSION

Findings

Brucella melitensis isolated from Ulanqab of