Rapid differentiation between E. coli and Salmonella Typhimurium using metal oxide sensors integrated with pattern recognition

Abstract

Abstract A rapid method to differentiate between E coli and Salmonella Typhimurium was developed. E. coli and S. Typhimurium were separately grown in super broth and incubated at 37 °C. Super broth without inoculation of E. coli or S. Typhimurium was used as control. Numbers of E. coli and S. Typhimurium were followed using a colony counting method. Identification of the volatile metabolites produced by E. coli and S. Typhimurium was determined using solid-phase microextraction coupled with gas chromatography/mass spectrometry. An electronic nose with 12 non-specific metal oxide sensors was used to monitor the volatile profiles produced by E. coli and S. Typhimurium. Principal component analysis (PCA) and back-propagation neural network (BPNN) were used as pattern recognition tools. PCA was used for data exploration and dimensional reduction. PCA could visualize class separation between sample subgroups. The BPNN was shown to be capable of predicting the number of E. coli and S. Typhimurium. Good prediction was possible as measured by a regression coefficient ( R 2 = 0.96) between true and predicted data. Using metal oxide sensors and pattern recognition techniques, it was possible to discriminate between samples containing E. coli from those containing S. Typhimurium.