Abstract
The method of rapid identification of fish flavobacteriosis using polymerase chain reaction (PCR) was developed. Specifity and efficiency of the selected oligonucleotide primers was confirmed. Nine Flavobacterium strains were isolated from clinically healthy and infected with Flavobacteriosis rainbow trout Onchorhynchus mykiss. Flavobacterium columnare strain was identified using the developed rapid diagnosis method. Phylogenetic analysis had indicated the kinship of isolated strains to Flavobacterium genera. Neither F. psychrophylum or F. branchiophilum strains were identified in the isolated samples. The species identification capacity using the 16S rRNA gene restriction analysis was shown. The developed PCR method can be used for rapid identification of different fish flavobacteriosis forms in fish farms in Ukraine.
Highlights
The genus Flavobacterium is characterized with gram-negative, aerobic, oxidasepositive, nonenzymatic, yellow-pigmented and mostly gliding bacteria that do not form spores [1]
Bacteria F. psychrophylum is the agent of infectious disease of salmon called “Bacterial cold water disease” (BCWD) [8] and cause liver inflammation in juvenile rainbow trout fish (Rainbow trout fry disease, RTFS) [9]
At meat peptonic agar (MPA) flavobacteria were shaped in a bright yellow colonies of different morphology
Summary
The method of rapid identification of fish flavobacteriosis using polymerase chain reaction (PCR) was developed. Bacteria F. psychrophylum is the agent of infectious disease of salmon called “Bacterial cold water disease” (BCWD) [8] and cause liver inflammation in juvenile rainbow trout fish (Rainbow trout fry disease, RTFS) [9]. Columnaris or cotton-wool disease, caused by bacterial pathogen F. columnare – is infectious and highly contagious disease, that cause massive loss of freshwater fish species. This disease develops at a temperature of 15 °C and above and is found in salmon, channel catfish, carp and others [11]. The purpose of given research was to isolate bacteria of Flavobacterium genus from infected and clinically healthy rainbow trout Onchorhynchus mykiss and develop a rapid diagnostic method of fish flavobacteriosis using polymerase chain reaction (PCR)
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