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Abstract
Clinical strains of Yersinia.pseudotuberculosis were screened for 70 Kbp virulence plasmids it was found only 20 % carrying 70Kbp plasmid, when subjected to different concentrations of Naledixin for development of mutant strains , plasmids were lost from one strain Yersinia pseudotuberculosis IP3295, which leaded to the modification of L.B medium into human blood (H.B) L.B medium as culture medium for Yersinia pseudotuberculosis .Yersinia pseudotuberculosis IP3295 showed 70Kbp virulence plasmid with additional small plasmids which proves the incorporation of plasmids with chromosomal DNA and similarity with Yersinia pestis. All strains of Yersinia pseudotuberculosis harboring virulence plasmids were detected for FyuA and Irp2 genes of the High pathogenicity island using PCR. And it was found that all plasmid harboring strains contains high Pathogenicity Island.
Highlights
Yersinia spp. are gram-negative, facultative rods that are motile when isolated from environment but become non-motile in mammalian host
Clinical strains of Yersinia.pseudotuberculosis were screened for 70 Kbp virulence plasmids it was found only 20 % carrying 70Kbp plasmid, when subjected to different concentrations of Naledixin for development of mutant strains, plasmids were lost from one strain Yersinia pseudotuberculosis IP3295, which leaded to the modification of L.B medium into human blood (H.B) L.B medium as culture medium for Yersinia pseudotuberculosis .Yersinia pseudotuberculosis IP3295 showed 70Kbp virulence plasmid with additional small plasmids which proves the incorporation of plasmids with chromosomal DNA and similarity with Yersinia pestis
20 clinical strains of Yersinia pseudotuberculosis were screened for virulence plasmids, and only 5 strains harboring virulence plasmids, and after treatment with naledixin to be naledixin mutants, still harbouring the 70 Kbp virulence plasmids
Summary
Yersinia spp. are gram-negative, facultative rods that are motile when isolated from environment but become non-motile in mammalian host. Three species of Yersinia cause disease in humans: Yersinia enterocolitica, Yersinia pseudotuberculosis, and Yersinia pestis. Infections due to Y. enterocolitica and Y. pseudotuberculosis are acquired by ingestion of Contaminated food or water Both of these species are ubiquitous in the environment and infect a variety of wild and domestic animals as well as humans. Y. enterocolitica gastrointestinal infections are followed by arthritis of the peripheral joints, which develops 2 to 6 weeks after the intestinal infection has cleared. This arthritis is called reactive arthritis; because it is thought to be caused by T cells and / or antibodies elicited by antigens that cross-react with host antigen, not by bacterial infection of the joints. (or ) T cells that recognize B27 could attack host cells (Fukushima et al , 2001; Denecker et al, 2002; Hayashidani et al, 2002; Juris et al, 2002)
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