Abstract
Two commercial methods for diagnosing acute Epstein-Barr virus (EBV) infections were evaluated. The methods compared included detection of heterophile antibodies with bovine erithrocyte antigens-sensitized latex particles and a rapid ELISA method which differentiates IgG and IgM responses against a synthetic peptide (p62). This is an antigenic portion of the EBV nuclear antigen. A total of 314 serum samples was used in the study, including sera from EBV acute infections (249), cytomegalovirus infections (11), and acute rubella (9), as well as sera from healthy pregnant women (25) and normal blood donors (20). With sera from EBV infections, we obtained sensitivity values ranging from 65·1% (heterophile antibodies by latex agglutination) to 70·3% (IgM to p62 by ELISA). In children below five, the ELISA method showed the highest sensitivity. Among cytomegalovirus patients, as well as in healthy people, some false-positive results were obtained by the ELISA method. The results obtained confirmed that virus specific serological markers are often necessary for the diagnosis of EBV infections.
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