Abstract
We present a one-step inhibition biosensor method that can be used to determine mixtures of three organophosphorus compounds. We propose to calculate the rate of irreversible inactivation of the enzyme (kp) to demonstrate the direct relationship between enzymatic biosensors and Chlorpyriphos-oxon (CPO), Chlorfenvinphos (CFV) and Azinphos-methyl oxon (AZMO) insecticides. The array of biosensors was designed using only two acetylcholinesterases from Drosophila melanogaster (wild-type and genetically modified). We used the values the slope of inhibitions mi from an array of biosensors and an artificial neural network to solve mixtures of CPO, CFV and AZMO insecticides The errors were low between the obtained and expected values for a set of 20 external test samples.
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