Rapid determination of metanil yellow in turmeric using a molecularly imprinted polymer dispersive solid-phase extraction and visible light spectrophotometry

Abstract

The present study aimed to develop a sensitive and available method for determining metanil yellow (MY) as an adulterating agent in food samples. Solid-phase extraction was chosen for pre-concentrating metanil yellow prior to its determination using a validated UV-spectrophotometric method. The precipitation polymerization method was applied to synthesize a range of molecularly imprinted polymers (MIPs) for selective extraction of MY. Polymers were characterized by SEM and FTIR and investigated for MY extraction through batch rebinding experiments. The extraction process was optimized in the term of pH, time, capacity, and the desorbing solvent. Results of this study showed the critical role of template/functional monomer ratio in the preparation of the MIPs. The developed MIP solid-phase extraction/UV-spectrophotometric method was employed for determining MY in spiked samples and showed 88.10–92.76% recovery for turmeric samples containing 0.1–10 mg/kg MY. The developed method was shown selective for MY in the presence of another azo dye.