Rapid Determination of Ciprofloxacin in Cerebrospinal Fluid by Micellar Electrokinetic Chromatography with Direct Sample Injection and its Application in Tuberculosis Meningitis

Abstract

A micellar electrokinetic chromatography (MEKC) method with ultraviolet detection at 280 nm is described for analysis of ciprofloxacin in cerebrospinal fluid (CSF) by direct injection without sample pretreatment. The proposed method was applied in one tuberculosis meningitis patient monitoring the concentration of ciprofloxacin in CSF after oral administration of 500 mg of ciprofloxacin after 12 hr. The separation of ciprofloxacin from the CSF matrix was performed at 25°C using Tris buffer with sodium dodecyl sulfate (SDS) as the background electrolyte. Using moxifloxacin as an internal standard (IS), the linear range of the method for the determination of ciprofloxacin in CSF was between 0.1–5.0 µg/mL; the detection limit of the ciprofloxacin in CSF (signal to noise ratio = 3; injection 1.0 psi, 25 s) was 50 ng/mL.