Abstract
Cell volume density was shown to be a useful growth parameter to monitor both single cell and aggregate suspension cultures. A method is described to quickly and easily measure this parameter using the length of a cell pellet in a conical hematocrit tube. The tubes were prepared by sealing the small end of disposable micropipet tips, and for seven cell lines growth within the range from 0.1 to 5 ml/liter was reproducible with an average ± 5.6% SD. Good agreement was obtained for population doubling times determined from cell volume density, DNA concentration, and, where possible, cell number density.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
