Abstract
A rapid UPLC-MS/MS method was developed for the quantitative determination of anisatin, as a marker of the sesquiterpene lactones in various Illicium species, in plasma, urine, vomit, and plant samples using a triple quadrupolep mass spectrometer. The proposed assay includes multi-function impurity adsorption SPE cleanup procedure for plasma sample, diatomite solid supported liquid/liquid extraction for urine, and tert-butyl methyl ether liquid/liquid extraction for plant sample. The satisfactory recoveries were obtained. Chromatographic separation was performed on an UPLC BEH C-18 column (50 mm x 2. 1 mm x 1. 7 mu m) using gradient elution with water-methanol, and detected by the negative electrospray ionization-MS/MS method under multiple reaction monitor mode. The cycle time of each analysis was 5. 0 min. The average recoveries were 92. 6% - 100. 3% and 101% - 118% with RSDs of 3. 8% - 11% and 6. 4% - 17% (n=6) for anisatin in plasma and urine, respectively. The limits of quantitation (S/N=10) were 2 mu g/L and 1 mu g/L for plasma and urine sample, respectively. The method is simple, selective and sensitive to the determination of anisatin in biological sample for toxicological purposes.
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