Rapid determination of 55 fungicides in siraitia grosvenoriis by QuEChERS and high performance liquid chromatography-tandem mass spectrometry

Abstract

A method for the determination of 55 fungicides in siraitia grosvenoriis by QuEChERS and high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was established. The samples were extracted with acetonitrile containing 1%(v/v) acetic acid, then dehydrated by anhydrous magnesium sulfate and purified by anhydrous sodium sulfate, N-propyl ethylenediamine (PSA) and C18. Then, 95% (v/v) acetonitrile aqueous solution (organic phase) and water (aqueous phase), both containing 0.005 mol/L ammonium formate and 0.01% (v/v) formic acid, were used as mobile phases with gradient elution. The compounds were tested by positive electrospray ion source (ESI+) and dynamic multiple reaction monitoring (DMRM) mode. Fifty-five fungicides were quantified by external standard method considering matrix effects. The correlation coefficients (R2) were greater than 0.99, which indicated that the linear dependence of the 55 fungicides were good in the range of 1.0-100.0 μg/kg. The LODs (S/N>3) and LOQs (S/N>10) of all the fungicides were 1.0 μg/kg and 10.0 μg/kg, respectively. The recoveries were in the range of 76.96%-118.45% and the relative standard deviations (RSDs) ranged from 3.44% to 19.63% (n=6). This method has the advantages of rapidity, accuracy and sensitivity for the high-throughput detection of fungicides residues in siraitia grosvenoriis.