Abstract
Fusarium head blight disease (FHB) in wheat, caused by Fusarium graminearum species complex (Fg complex), is a very serious disease threatening wheat production worldwide. Polymerase chain reaction (PCR) based methods have been established for rapid and quantitative detection of many plant pathogens. In this study, a specific pair of primers was designed based on the sequence of DNA fragment (740 bp) amplified by a microsatellite primer M13 from Fg complex isolates. This pair of primers was able to amplify a 380 bp fragment from all Fg complex isolates but not from any other tested fungal species. Using this pair of primers, a real-time PCR assay was developed to quantitatively detect small amounts of Fg complex in wheat seeds. This sensitive and quantitative detection assay could be useful in epidemiological studies and assessment of mycotoxin contamination in wheat seeds.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
