Rapid detection of two markers of acute aortic dissection based on a lateral flow assay of hollow CdTe/CdS nanospheres

Abstract

Acute aortic dissection (AAD) is an emergency disease with a high mortality rate, and its characteristics is variable clinical symptoms, complex pathology, high misdiagnosis rate, leading to its frequent confusion with other chest pains of myocardial infarction and chronic emphysema in clinic; Therefore, early diagnosis and treatment of acute aortic dissection is the key to reduce its mortality and improve the prognosis of patients., and timely & rapid diagnosis is crucial for successful treatment. Conventional methods (such as PCR, MRI, and ELISA) cannot be implemented as a rapid point-of-care (POC) test for ADD. Highly fluorescent hollow CdTe/CdS nanospheres (NSs) with the inside size of ∼240 nm and shell thickness of ∼19 nm were prepared via the hydrothermal method, and three color NSs with green, yellow and red fluorescence of wavelength of 535 nm, 580 nm, and 620 nm, respectively, were obtained by changing the preparation temperature. Immunochromatographic (ICG) assay were developed with the prepared yellow CdTe/CdS NSs for the sensitive and qualitative detection of high mobility group box protein B1 (HMGB1). Visual limit of detection of ICG for HMGB1 antigen was 3.13 ng/mL within 15 min, lower than that of normal ELISA. Furthermore, a multichannel lateral flow assays (mLFA) was developed with green and yellow CdTe/CdS NSs for the simultaneous, specific, sensitive, and qualitative detection of HMGB1 and soluble growth stimulating gene 2 (sST2), and visual limits of detection of HMGB1 and sST2 in mLFA were 10 ng/mL and 1.25 ng/mL, respectively. This mLFA of CdTe/CdS NSs with two marker-antibodies first facilitates its application of POC in the early diagnosis on AAD.