Rapid detection of tulathromycin in pure milk and honey with an immunochromatographic test strip

Liqiang Liu,Liguang Xu,Steven Suryoprabowo,Shanshan Song,Hua Kuang

doi:10.1080/09540105.2017.1376040

Abstract

ABSTRACT In this paper, we describe the development of a rapid, simple, specific, and sensitive immunochromatographic strip test based on an antibody–antigen reaction for detection of tulathromycin (TULA) in samples of 0.01 M PBS pH 7.4, pure milk and honey. The monoclonal antibodies specifically recognized the corresponding antigens produced in our laboratory. Under optimized conditions, the cut-off limits of the test strips for TULA in 0.01 M PBS pH 7.4 was found to be 2.5 ng/mL. Meanwhile, with simple preparation for detection in pure milk and honey samples, the cut-off limits for TULA were found to be 5 and 10 ng/mL, respectively. Each test can be evaluated in 5 min. In summary, this lateral-flow device provides a sensitive, effective, specific, and rapid method for detection of TULA residues in food matrix.

Highlights

Tulathromycin (C41H79N3O12, M: 806.08 g/mol; TULA) (Figure 1), is a novel member of the triamilide subclass of macrolide antibiotics, which is used for the treatment of bacterial respiratory diseases of cattle and swine
In order to function as the reaction matrix in an immunochromatographic assay, the strip materials must be hydrophilic and have consistent flow characteristics
Many factors affect the binding process and these must be considered when developing assays and processing NC membranes. Some of these factors are: (1) reagent choices: non-specific proteins such as bulking proteins (BSA, casein) compete for binding sites, materials that interfere with hydrophobic interactions, and polymers such as polyvinyl acetate, polyvinylpyrrolidone (PVP), and polyethylene glycol (PEG) and (2) environment humidity should be optimized for binding (25–50% relative humidity at room temperature)

Summary

Introduction

Tulathromycin (C41H79N3O12, M: 806.08 g/mol; TULA) (Figure 1), is a novel member of the triamilide subclass of macrolide antibiotics, which is used for the treatment of bacterial respiratory diseases of cattle and swine. A single dose of TULA demonstrates high clinical effectiveness after intramuscular injection. TULA is rapidly absorbed from the injection site with great bioavailability and a long elimination half-life of 4–6 days. Pro-longed exposure of lung tissues to macrolides are important for the treatment and prevention of bovine respiratory disease. The incorrect use of TULA may leave residues in edible tissues, milk, and honey, and resulting in undesirable effects on consumer health such as the onset of allergies in individuals with hypersensitivity and the appearance of antibiotic-resistant bacteria (Hoogenboom et al, 2008; Hurd, 2004)

Objectives

Methods

Results