Rapid detection of nitrite based on nitrite-oxidizing bacteria biosensor and its application in surface water monitoring

Abstract

Timely and sensitive detection of nitrite is of great significance for human health protection and water pollution treatment. However, many biosensors can only determine the comprehensive toxicity of the water, and there are few electroactive biofilm (EAB) sensors for the specific detection of pollutants. Biofilms formed by bacteria with specific functions can improve the specificity of nitrite identification by biosensors. This study developed a novel, rapidly responding, high sensitivity (958.6 μAμM−1cm−2), wide detection range and anti-interference electrochemical biosensor based on electroactive nitrite-oxidizing bacteria. The biosensor could accurately detect nitrite in the range of 0.3–100 mg/L within 3 min by the cyclic voltammetry (CV) method. The bioelectrode could perform stable detection of nitrite over 200 cycles. The specificity of the biosensor for detecting nitrite was demonstrated by the presence of nitrite oxidizing bacteria (NOB) and nitrite oxidase enzyme (NXR) on the electrode biofilm. The biosensor performed well in wetlands and rivers, with an RSD <14.8% in the detection of nitrite at low concentrations, and further revealed the nitrification occurrence. Our study provided a feasible way for the development of a highly sensitive, rapidly responding and stable electrochemical biosensor, which also exhibited potential applications for long-term detection of nitrite and assessment of ecological function in surface water (rivers, lakes, wetlands, marshes, etc.).