Abstract
Loop-mediated isothermal amplification was employed for the detection of fowl adenoviruses as a simple, rapid and field based diagnostic assay. Out of 134 field samples screened, 28 samples were positive by both PCR and LAMP assay. The LAMP assay primers were found to be highly specific and it detected only fowl adenovirus samples and it did not react with other avian viruses like Marek’s disease virus and avian leucosis virus. Conventional PCR detected 100ug level of DNA whereas LAMP assay detected up to 10ng level of DNA. It clearly indicated that LAMP assay was 100-times more sensitive than conventional PCR. The specificity of LAMP assay was confirmed by sequencing of the LAMP amplified products and real time PCR. The LAMP positive reaction can be easily visualized under UV trans- illuminator by the addition of SYBR green dye. Thus, LAM Passay proves to be a sensitive, rapid, less-laborious and cost- effective technique for the diagnosis of fowl adenoviruses in field conditions.
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