Abstract
AbstractIn this study, the surface‐enhanced Raman spectroscopy (SERS) method was applied to determine florfenicol residues (FF) in chicken meat. Gold nanoparticles were stabilized on the glass slides cost‐effectively by “1‐dodecanethiol.” Three factors, including “solvent types,” “spectroscopy times,” and “ ratio of gold nanoparticles and samples” were examined to optimize the spectrum acquisition. The theoretical Raman spectrum of FF was calculated to compare the experimental spectrum (Asymp. Sig. = 0.0655). FF fingerprint was determined in solid and water‐soluble states, and the index peak assignment was performed. In the quantitative measurements of FF, a value of LOD = 0.25 μg/L was obtained, and its linear regression equation was calculated. In the field activities, FF was added to chickens drinking water (including five dosages in three replications), and FF residues were determined by the SERS and LC/MS/MS methods. The results showed no statistically meaningful differences (Asymp. Sig. = 0.714, p < .01).
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