Abstract
A rapid method for the sensitive detection of bacteria using magnetic immunoassay, which are measured with a tunneling magnetoresistance (TMR) sensor, is described. For the measurement of Escherichia coli O157:H7 (E. coli O157:H7) bacteria, the target was labeled by magnetic beads through magnetic immunoassay. The magnetic beads produce a weak magnetic fringe field when external field is applied, thus induce the magnetoresistance change of TMR sensor. A detection limit of 100 CFU/mL E. coli O157:H7 bacteria in 5 hours was obtained. With its high sensitive and rapid detection scheme based on the TMR biosensor, the detection system is an excellent candidate suitable and promising for food safety and biomedical detection.
Highlights
The E. coli O157:H7 bacteria is detected in the range of 105 to 1010
The minimum concentration of 100 colony-forming units (CFU)/mL for E. coli O157:H7 bacteria can be detected in 5 hours
The biosensor fabricated based on tunneling magnetoresistance (TMR) sensor is successfully applied for the detection of E. coli O157:H7 bacteria
Summary
The high sensitivity and the short assay time was obtained.[15] In this perspective, even low concentration of magnetic immunoassay can be detected, which is effective way to shorten pre-treatment time or replace enrichment steps. It is paving the way for rapid detection of E. coli O157:H7. Magnetic beads labeled on TMR sensor directly, will cause the high test cost and the slow detection speed. The minimum concentration of 100 CFU/mL for E. coli O157:H7 bacteria can be detected in 5 hours
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